Tunable dual-AAV sparse labeling of PV⁺ retinal ganglion cells enables single-neuron projection by fMOST.

ABSTRACT:

Introduction

Sparse and bright labeling of retinal ganglion cell (RGC) is essential for correlating single-cell morphology with brain-wide visual circuitry. This study aimed to develop a cell-type-specific, sparse labeling strategy for parvalbumin-expressing RGCs (PV⁺ RGCs) in the transgenic mouse retina using recombinant adeno-associated virus (rAAV) and to map the whole-brain projection patterns of single PV⁺ RGCs via fluorescence micro-optical sectioning tomography (fMOST).

Methods

A cell-type-specific dual AAV system was employed, co-packaging a Cre-dependent Flpo plasmid and an Flpo-dependent enhanced yellow fluorescent protein (EYFP) plasmid. Key parameters-including the mixing ratio of core plasmids (ranging from 1/100 to 1/1000), gene copy numbe

SUBMITTER: Zhou L 

PROVIDER: S-EPMC12833038 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature