Integrative transcriptomic analysis reveals microglial metabolic-inflammatory crosstalk of HK2-HSPA5-TNF axis after intracerebral hemorrhage.

ABSTRACT:

Background

Intracerebral hemorrhage (ICH) triggers secondary brain injury through neuroinflammation, yet the interplay between metabolic reprogramming and inflammatory responses remains poorly defined. This study investigated how glucose metabolism dysregulation contributes to neuroinflammatory pathogenesis following ICH.

Methods

We integrated transcriptomic datasets from bulk RNA sequencing (human perihematomal tissue), single-cell RNA sequencing (mouse ICH model), and spatial transcriptomics (mouse time-series). Bioinformatic analyses included differential expression screening, single-cell weighted gene co-expression network analysis, pseudotemporal trajectory reconstruction, and cell-cell communication inference to identify key metabolic-inflammation regulators and their

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12833071 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature