Characterization of the biodistribution profile of a human Dialyzable Leukocyte Extract (hDLE) by in vivo fluorescence imaging: a strategy to infer the ADME profile of complex multipeptide drugs.

ABSTRACT: The immunomodulator Transferon Oral^® (TO) is a human Dialyzable Leukocyte Extract (hDLE), id est, a complex mixture of peptides smaller than 10 kDa derived from human buffy coats. TO reduces TNF-α, IL-6, and IgE; and induces IFN-γ levels, which makes it useful in the treatment of allergies, autoimmunity, and infections. Its complex composition has made it difficult to characterize its pharmacokinetic profile, target organs, and mechanism of action. This study focused on describing the biodistribution profile of TO peptides indirectly using in vivo imaging. The TO peptides were coupled to the fluorophore Alexa 488 and administered intravenously (IV), subcutaneously (SC), intraperitoneally (IP), intramuscularly (IM), and oropharingelly (ORO) to female and male Crl: N

SUBMITTER: Fragozo A 

PROVIDER: S-EPMC12833350 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature