Trajectory analysis reveals an uncommitted neuroblastic state in MYCN-driven neuroblastoma development.

ABSTRACT:

Background

Understanding the factors that determine the spontaneous regression of pre-cancerous lesions is critical to advancing cancer prevention. Neuroblastoma, a pediatric cancer, undergoes spontaneous regression more frequently than other types of cancer.

Methods

Here, we analyzed the transcriptomic features of spontaneous regression in pre-cancerous neuroblasts using Th-MYCN mice, an animal model that closely resembles human neuroblastoma. Single-cell transcriptomic analysis of ganglion tissues from Th-MYCN mice was conducted to elucidate the cellular and molecular underpinnings.

Results

Trajectory analysis of pre-cancerous neuroblasts revealed a distinct subtype we designated as "uncommitted" cells, characterized by the expression of neuronal genes, indicative o

SUBMITTER: Tsubota S 

PROVIDER: S-EPMC12833547 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature