Robust, scalable and xeno-free protocol for differentiating human induced pluripotent stem cells into functional macrophages.

ABSTRACT: Human induced pluripotent stem cells (hiPSCs)-derived macrophages (iMacs) exhibit key macrophage phenotypic and functional properties, positioning them as promising candidates for allogenic cell immunotherapies. However, an efficient, scalable and good manufacturing practices (GMP)-compatible differentiation protocol is noticeably lacking. To meet this need, we aimed to develop a robust protocol for differentiating clinical-grade hiPSC lines into functional iMacs, designed for scalability and immediate GMP translation. We tested different media compositions, cytokine concentrations, seeding densities, culture regimens (2D vs. 3D) and coatings across key developmental stages. With the optimized protocol, we differentiated three different clinical-grade hiPSC lines towards mesoderm through e

SUBMITTER: De Homdedeu M 

PROVIDER: S-EPMC12833622 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature