Insights into the colloidal and structural stability of Atezolizumab with nonpolar amino acid-based ionic liquids under multiple stresses: phase two.

ABSTRACT: Monoclonal antibodies (mAbs) are susceptible to physical and chemical instabilities, which compromise their therapeutic efficacy and shelf life. This study investigates the potential of renewable choline-amino acid-based ionic liquids (Ch-AA ILs), specifically choline valinate (CV) and choline glycinate (CG), to enhance the stability of the aglycosylated IgG1 antibody, Atezolizumab (Amab). Amab was formulated in varying concentrations of the ionic liquids (ILs) and subjected to ambient, thermal (40-70 °C), and chemical (urea) stress conditions. Stability was assessed using Dynamic Light Scattering (DLS) to measure the hydrodynamic diameter (D _r), UV-visible spectroscopy to determine the Aggregation Index (AI), and Attenuated Total Reflectance-Fourier Transform Infrared sp

SUBMITTER: Alkhawaja B 

PROVIDER: S-EPMC12833817 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature