Transcriptomic insights into cultivation-driven virulence in Aeromonas spp.: a new approach to optimizing autogenous vaccines in aquatic veterinary medicine.

ABSTRACT: Autogenous vaccines are a critical tool in aquaculture for managing bacterial diseases when commercial vaccines are unavailable or ineffective. To improve vaccine efficacy, this study explored how different cultivation conditions influence virulence gene expression in two major fish pathogens, Aeromonas salmonicida subsp. salmonicida and Aeromonas hydrophila. Isolates were cultured in nutrient-rich (tryptic soy broth [TSB]) and nutrient-limited (Mueller-Hinton broth [MH]) media, with and without supplementation of 1% fetal bovine serum (tryptic soy broth supplemented with 1% FBS [TSB1] and Müller-Hinton supplemented with 1% FBS [MH1]), to mimic environmental and host-like conditions. Total RNA was sequenced using the Oxford Nanopore MinION platform, and gene expression was quantified using

SUBMITTER: Zhao D 

PROVIDER: S-EPMC12833933 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature