MUC1 peptide-loaded dendritic cell vaccine boosts antitumor immunity in pancreatic cancer.

ABSTRACT:

Objective

Pancreatic cancer is one of the most aggressive malignancies with a poor prognosis and limited treatment options. This study aimed to evaluate the efficacy of a dendritic cell (DC) vaccine pulsed with mucin 1 (MUC1) peptide antigens in the immunotherapy of pancreatic cancer.

Methods

Mononuclear cells were isolated from umbilical cord blood and induced to differentiate into DCs. The surface markers of DCs and their phagocytic capacity for FITC-OVA were detected using flow cytometry. The stimulatory effect of DC vaccines loaded with MUC1 antigen peptides (568 and 619) on T lymphocyte proliferation was assessed by CCK-8 assay. ELISA was used to measure the secretion of IL-12p70 by DCs and IFN-γ production by activated cytotoxic T lymphocytes (CTLs). The proportion of

SUBMITTER: Xie H 

PROVIDER: S-EPMC12834808 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature