Advancing urine-derived stem cells: Cryopreservation validation and sex-specific metabolism.

ABSTRACT:

Background

Urine-derived stem cells (UDSC) are an emerging, non-invasive source of human stem cells combining easy collection, broad accessibility and high patient compliance with multilineage differentiation capacity. However, key gaps remain in UDSC research, particularly in understanding sex-related differences and the lack of a validated cryopreservation protocol, a critical aspect for primary cells, given their variability in colony formation, proliferation rates and experimental timing. To address these limitations, this study aimed to establish, for the first time, a reliable protocol for UDSC cryopreservation and to explore potential sex-related differences, with a specific focus on glycolysis and mitochondrial respiration.

Methods

UDSC were isolated from urine sampl

SUBMITTER: Afonso GJM 

PROVIDER: S-EPMC12834848 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature