Temperature downshifts induce biofilm formation in Pseudomonas aeruginosa through the SiaABCD signal and functional module.

ABSTRACT: Pseudomonas aeruginosa is a highly adaptable Gram-negative pathogen known for its remarkable ability of forming biofilms. Understanding the environmental cues and regulatory mechanisms that drive biofilm formation is essential for developing effective control strategies. In this study, we screened 57 clinical and environmental P. aeruginosa isolates and discovered that a universal environmental cue, temperature downshift from host-associated 37 °C to room temperature (21 °C), significantly promotes biofilm formation in 63% of the strains. Using the ATCC 27853 strain as a model, we demonstrate that this enhancement results from increased production of the Psl exopolysaccharides at lower temperature. LC-MS/MS analysis revealed elevated levels of the secondary messenger c-di-GMP, a key regula

SUBMITTER: Li Y 

PROVIDER: S-EPMC12834913 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature