Self-assembling multilayer MSC-sheet promotes wound healing increasing M2 macrophage polarization.

ABSTRACT: Mesenchymal stem cell (MSC) therapy holds promise for treating chronic wounds. However, low cell density in the target tissue and short retention time limits its efficacy. This study investigated the therapeutic effects of the self-assembling multilayer MSC-sheet in a murine wound model, focusing on wound healing and macrophage infiltration. The MSC-sheet was prepared from human subcutaneous adipose tissue and characterized by histology and RNA sequencing. In vivo, the α-MEM (control), MSC-suspension, and MSC-sheet were applied to full-thickness skin defects in C57BL/6 J Jcl mice. On days 7, 14, and 21, the remaining wound area, neoepithelialization, granulation tissue, cell retention time, angiogenesis, and macrophage infiltration were evaluated using hematoxylin and eosin, Azan, anti-hum

SUBMITTER: Zhao Q 

PROVIDER: S-EPMC12834999 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature