Expression and function of CYP enzymes and hepatobiliary transporters in an improved long-term sandwich culture hepatocyte model.

ABSTRACT: Recent advances in methodology help long-term in vitro maintenance of primary human hepatocytes (PHH), improving their predictability for drug-induced liver injury. Small-molecule additives, such as 5C, have been described to prolong hepatocyte functionality, however, comprehensive description of CYP enzyme and transporter functions are scarce. Here we show a new hepatic model developed by treating hepatocytes in sandwich configuration with 5C (SPHH-5C), where, besides keeping hepatocytes in a fully differentiated form, their polarization is also supported to achieve more in vivo-like structure. The model is characterized by CYP enzyme and hepatobiliary transporter expression and activity as a function of culture time. Hepatic functions were compared in untreated (SPHH) and 5C-treated sand

SUBMITTER: Pacsuta J 

PROVIDER: S-EPMC12835129 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature