Integrative biochemical and computational analysis of leflunomide-induced redox alterations in Nauphoeta cinerea.

ABSTRACT: Leflunomide (LF) is an immunomodulator classified as a disease-modifying anti-rheumatic agent. Its mechanisms of action include inhibition of dihydroorotate dehydrogenase in lymphocytes, resulting in immunosuppressive effects, and blocking tyrosine kinase activation, generating anti-inflammatory effects. This study aimed to study biochemical parameters 24 h after leflunomide administration to Nauphoeta cinerea (Cockroach) model, focusing on toxicity assessment. Biochemical analyses were performed, and molecular docking studies were conducted with the proteins 6VCD, 2B3X, and 2B3Y. Leflunomide altered biochemical parameters by increasing markers such as PSH, NPSH, TBARS, and iron levels, suggesting that LF may induce oxidative stress. This was supported by a dose-dependent increase in PSH,

SUBMITTER: Dos Santos CAL 

PROVIDER: S-EPMC12835159 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature