GPR88 promotes neurite outgrowth of sensory neurons via activation of G_i/o.

ABSTRACT:

Introduction

The peripheral nervous system retains the intrinsic ability to regenerate: after nerve injury, axons can sprout and reinnervate their target organ. We hypothesized that this regenerative response was driven by a transcriptional program involving druggable gene targets that promote neurite outgrowth.

Methods

Sensory neurons were isolated from rat dorsal root ganglia (DRG) and cultured in vitro. Transcript levels were determined by sequencing of RNA. RNAscope was used to visualize GPR88 transcripts in individual subtypes of DRG neurons. Neurite outgrowth was quantified in the absence and presence of allosteric activators of GPR88 to generate concentration-response curves. Neuronal cultures were incubated in the presence of pertussis toxin and gallein to block Gi/G

SUBMITTER: Un D 

PROVIDER: S-EPMC12835199 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature