Enhancing gougerotin production by screening endogenous promoters for the transporter gene gouM in Streptomyces albulus CK-15.

ABSTRACT:

Introduction

Gougerotin is a nucleoside antibiotic that exhibits strong inhibitory effects against bacteria, as well as activities against plant viruses and pathogenic fungi, making it highly valuable for development and application. However, its widespread use is limited by low production yield and long fermentation time. In the gougerotin biosynthetic gene cluster, the gouM gene is a transporter gene, and its encoded protein is responsible for transporting gougerotin to the extracellular space.

Methods

To validate the impact of the gouM gene on gougerotin production, we generated a mutant strain by knocking out the gouM gene. To enhance the expression level of the gouM gene and thereby improve its ability to transport gougerotin, we screened end

SUBMITTER: Liu B 

PROVIDER: S-EPMC12835213 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature