Integrated enzymatic and sonication strategy for sustainable soybean processing: from cell wall deconstruction to product separation.

ABSTRACT: Conventional soybean processing relies on hexane extraction and high-temperature treatments, which cause protein denaturation and hinder the separate recovery of oil, protein, and carbohydrate. To address this limitation, this study aimed to establish a sustainable enzymatic soybean processing (ESP) strategy for separate collection of all three major components: intact oil bodies, undenatured protein, and monomerized carbohydrate. This is the first demonstration that ESP efficiency can be significantly enhanced by integrating pulsed sonication. Multi-enzyme systems were produced via solid-state fermentation (SSF) of soyhull by Aspergillus niger and applied to cracked soybean particles. Screening of 15 SSF enzyme extracts revealed that pectinase, polygalacturonase, and invertase were the li

SUBMITTER: Ovi AQ 

PROVIDER: S-EPMC12835480 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature