Targeting the NEK7/NLRP3 Inflammasome Axis: Synergistic Protection of Intravitreal MCC950 and Systemic Metformin Against Diabetic Retinopathy in Rats.

ABSTRACT:

Objective

Diabetic retinopathy (DR) is characterised by chronic neuroinflammation where the NLRP3 inflammasome plays a pivotal role. This study investigated the therapeutic potential and underlying mechanism of combining systemic metformin (MET) with intravitreal MCC950, a specific NLRP3 inhibitor, in a rodent model of DR.

Methods

A type 2 diabetic rat model was induced by high-fat diet and streptozotocin (STZ) injection. Diabetic rats were divided into DR, MET, MCC950 and MET+MCC950 treatment groups. Body weight and blood glucose were monitored. Retinal structural changes were assessed by HE and PAS staining. Apoptosis was detected by TUNEL assay, and oxidative stress was evaluated by ROS fluorescence. The expression and interaction of key proteins within the NEK7/NLRP3 pat

SUBMITTER: Ren K 

PROVIDER: S-EPMC12835612 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature