Chromosome-scale genome assembly of Malcolmia littorea using long-read sequencing and single-pollen genotyping technologies.

ABSTRACT: Malcolmia littorea, a member of the family Brassicaceae, is adapted to coastal and sandy environments and has become a model in studies of reproductive barriers. However, genomic resources for the species are limited. Here, with the aim of understanding the molecular mechanisms underlying key traits in M. littorea, we present a de novo genome assembly consisting of 10 chromosome-scale sequences. We employed a high-fidelity long-read sequencing technology for genome assembly. To anchor the sequences to chromosomes, we developed a single-pollen genotyping method to construct a genetic linkage map based on SNPs derived from transcriptomes of pollen grains, possessing recombinant haploid genomes. We built a genome assembly consisting of 10 chromosome-scale sequences (215 Mb in total) for M. li

SUBMITTER: Shirasawa K 

PROVIDER: S-EPMC12836427 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature