Metabolically hijacked Listeria monocytogenes synergizes STING activation and dual cell death for enhanced antitumor immunotherapy.

ABSTRACT:

Background

Listeria monocytogenes (LM) holds promise as a microbial vector for cancer immunotherapy, yet its clinical translation requires precise engineering to achieve an optimal balance between attenuation and immunogenicity. This study aimed to develop an engineered LM strain that induces metabolic reprogramming while enhancing antitumor efficacy through multimodal immune activation.

Results

We encapsulated cepharanthine nanoparticles (CEP NPs) into a double-gene attenuated LM strain via electroporation, resulting in the induction of metabolic reprogramming and the emergence of a 'zombie-like' phenotypic state (LM^e@CEP). This modification disrupted folate metabolism to induce bacterial metabolic dormancy, while triggering a cyclic dinucleotide (CDN) surge that

SUBMITTER: Liu T 

PROVIDER: S-EPMC12836796 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature