Evaluation of the influence of RNA extraction methods on the detection of citrus viruses and viroids.

ABSTRACT:

Background

Certification and disease management practices are reliant on accurate pathogen detection assays. Effective nucleic acid extraction is the cornerstone of various molecular detection techniques, and many virus and viroid RNA extraction protocols have been developed, each with accompanying advantages and limitations. The extraction method influences the type, quantity and quality of RNA and, therefore, the overall sensitivity of the assay.

Methods

Plants from two citrus types were infected with multiple viruses and viroids. Ten plants from each were selected. RNA was extracted from bark and leaf samples using four protocols: CL (CTAB/LiCl), CE (CTAB/ethanol), TL (acid phenol buffer/LiCl and isopropanol) and SI (SDS/isopropanol). Extracts were subjected to RT-qPCR fo

SUBMITTER: Dippenaar M 

PROVIDER: S-EPMC12836986 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature