Prenatal lipopolysaccharide exposure programs the early-life lung development by modulating mesenchymal fibroblasts in a time- and dose-dependent manner.

ABSTRACT:

Background

Bronchopulmonary dysplasia (BPD) is one common and severe complication of preterm births. Prenatal infection/inflammation is the major cause driving preterm deliveries, but its contribution to the early-life lung development remains unclear.

Methods

Pregnant C57BL/6J mice were randomized at 15.5 or 18.5 days post coitum (dpc) to receive intraperitoneal injection of sterile saline, lipopolysaccharide (LPS) 50 μg/kg, or LPS 100 μg/kg. Animal experiments were performed compliantly to the ARRIVE guidelines. Lungs of newborn pups of mixed genders were harvested at the first postnatal day (P1) for histopathology to investigate prenatal LPS-induced structural changes. Expression of key pro- and anti-inflammatory mediators was assessed at both transcriptional and translat

SUBMITTER: Dong Y 

PROVIDER: S-EPMC12837034 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature