Aldolase a coordinates macropinocytic nutrient scavenging and lysosomal degradation in lung cancer by interacting with V-ATPase.

ABSTRACT:

Background

Oncogenic KRAS mutations in cancer cells drive macropinocytosis, a pathway that scavenges extracellular proteins to obtain nutrients under metabolic stress. Lysosomal degradation is essential for processing these proteins, but the precise molecular link between macropinocytic uptake and lysosomal processing remains largely unclear. Aldolase A (ALDOA), a glycolytic enzyme, has diverse non-canonical functions. While interactions between ALDOA and Vacuolar-type H⁺-ATPase (V-ATPase) subunits have been suggested, their functional role in lysosomal degradation of macropinocytosed cargo remains undefined.

Methods

We investigated the role of ALDOA using integrated approaches, including The Cancer Genome Atlas data analysis and in vitro assays of lung adenocarcinoma cell l

SUBMITTER: Kim D 

PROVIDER: S-EPMC12837057 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature