Two Engineered Bacillus subtilis Surfactin High-Producers: Effects of Culture Medium, and Potential Agricultural and Petrochemical Applications.

ABSTRACT: Two genetically engineered Bacillus subtilis strains, BMV9 and BsB6, were evaluated in terms of culture medium (effect of nutrients on surfactin yield) and potential biotechnological applications of surfactin in agriculture and the petrochemical industry. BMV9 (spo0A3; abrB*; ΔmanPA; sfp+) is, to date, the highest surfactin producer reported scientifically, and BsB6 is a sfp+ laboratory derivative strain that has also demonstrated considerable production potential. To assess their performance, fermentation experiments were conducted in shake flasks using two different culture media, a mineral salt medium and a complex medium, each supplemented with 2% (w/v) glucose. Lipopeptides (surfactin and fengycin) were extracted and quantified a

SUBMITTER: Correa GG 

PROVIDER: S-EPMC12837159 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature