Functional Characterization of ycao in Escherichia coli C91 Reveals Its Role in Siderophore Production, Iron-Limited Growth, and Antimicrobial Activity.

ABSTRACT:

Background

The emergence of antibiotic-resistant bacteria is one of the top health concerns. Escherichia coli is a Gram-negative bacterium that commonly causes severe infections. However, this research exposed its antibiotic-producing potential.

Methods

Rifampicin-resistant mutants of E. coli C91 were generated to activate cryptic BGCs. Mutants (C91-R1, R2 and R3) were tested for antimicrobial production using agar-well diffusion assays. Metabolite profiling was performed by LC-MS/MS. Siderophore production was tested by construction of a Δycao deletion mutant. Growth of this mutant was assessed under iron-limited conditions versus iron-rich conditions using dipyridyl. qRT-PCR was used to analyze gene expression entB, mcmA and mchF.

SUBMITTER: Dashti KM 

PROVIDER: S-EPMC12837419 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature