Application-Specific Measurement Uncertainty Software for Measuring Enrofloxacin Residue in Aquatic Products Using the Quick Quantitative (QQ) Method.

ABSTRACT: Quick Quantitative (QQ) immunoassays have been increasingly applied for the measurement of enrofloxacin (ENR) and ciprofloxacin (CIP) residues in aquaculture due to their speed and convenience. However, their quantitative reliability remains limited because measurement uncertainty (MU) is rarely considered during field testing. To enhance the metrological reliability of QQ-based residue analysis, we developed AquaUncertainty Pal, a mobile application that embeds real-time MU computation into the QQ workflow. The software automatically evaluates uncertainty sources during sampling and pipetting, visualizes the uncertainty budget, and guides users through optimized operations. The framework was validated against ISO/IEC 17025-accredited LC-MS/MS and assessed through a user study involving 20

SUBMITTER: Rong B 

PROVIDER: S-EPMC12837475 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature