Engineering Resilience: How Irradiation Strategies Influence 3D-Bioprinted Adipose Stem Cells.

ABSTRACT:

Background

Reconstructive defect coverage after irradiation remains a challenge in reconstructive surgery, as ionizing radiation leads to tissue ischemia and fibrosis. Therefore, the application of adipose-derived stem cells (ASCs) might be a therapeutic strategy for improving flap survival. Nevertheless, the influence of irradiation on ASCs remains unclear. This study examines the effect of irradiation on 3D-printed ASCs.

Methods

ASCs were 3D-cultured. The constructs were irradiated with 2 Gy and 5 Gy; one group treated with 0 Gy served as the non-irradiated control group. Cell viability was measured via a WST-8 assay, a live/dead assay and fluorescence microscopy 24 h, 48 h and 72 h after irradiation. Furthermore, qPCR analysis was performed to detect the expression of HIF

SUBMITTER: Amiri N 

PROVIDER: S-EPMC12837484 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature