Porcine FRZB (sFRP3) Negatively Regulates Myogenesis via the Wnt Signaling Pathway.

ABSTRACT: Secreted frizzled-related protein 3 (sFRP3/FRZB) is a soluble Wnt antagonist with established roles in skeletal development, however, its specific function in myogenesis remains underexplored. This study investigated the regulatory role of FRZB in muscle development, hypothesizing that it contributes to breed-specific growth differences in pigs. We examined FRZB expression in fetal tissues of slow-growing (Tibetan and Wujin) and fast-growing (Large White) pigs, and assessed its function in C2C12 myoblasts via siRNA-mediated knockdown. FRZB was widely expressed across porcine fetal tissues, with significantly higher abundance in the longissimus dorsi of slow-growing breeds. In vitro, FRZB silencing significantly enhanced myoblast proliferation and migratio

SUBMITTER: Nie J 

PROVIDER: S-EPMC12837581 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature