A highly conserved ABC transporter mediates cello-oligosaccharide uptake in the extremely thermophilic, lignocellulolytic bacterium <i>Anaerocellum bescii</i> (f. <i>Caldicellulosiruptor bescii</i>).

ABSTRACT: Cellulose deconstruction and utilization are foundational to renewable biofuel and biochemical production. Anaerocellum bescii (formerly Caldicellulosiruptor bescii) is an extremely thermophilic cellulolytic bacterium, notable for its multi-domain cellulases and hemicellulases that efficiently degrade lignocellulosic biomass. However, the mechanisms by which A. bescii transports cello-oligosaccharides released during cellulose degradation into the cell for catabolism remain unclear. Among its 23 ATP-binding cassette (ABC) sugar transporters, we identified a conserved ABC transporter locus (athe_0595-0598) encoding two extracellular binding proteins: Athe_0597 and Athe_0598. Biophysical analyses using differential scanning calorimetry and isothermal titration cal

SUBMITTER: Tjo H 

PROVIDER: S-EPMC12838199 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature