Involvement of <i>Escherichia coli-</i>encoded Lon protease and its substrates in phenotypic antibiotic resistance elicited by 4-amino-2-nitrophenol.

ABSTRACT: In prokaryotes, the energy-dependent protein degradation is controlled, primarily, by two ATP-dependent proteases, Lon and Clp. This study investigates the roles of Escherichia coli (E. coli)-encoded Lon protease in the metabolism of 2,4-dinitrophenol (2,4-DNP), a toxic industrial compound. Enhanced conversion of yellow-colored 2,4-DNP to a reddish-brown product was observed in a strain lacking Lon protease (Δlon). This observation led us to characterize and understand the mechanisms of 2,4-DNP metabolism. UV-visible and LC-MS analyses revealed differences in the conversion products between the wild-type and Δlon. One of the substrates of Lon protease is MarA, a transcription factor, and studies with different mutants followed by trans complementation dem

SUBMITTER: Nandini SS 

PROVIDER: S-EPMC12838266 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature