A reverse transcription-quantitative polymerase chain reaction system for evaluating intestinal butyrate production by fecal bacteria.

ABSTRACT: Intestinal butyrate is key in maintaining host health. As an indicator of butyrate production, the fecal butyrate concentration is used for assessing health status; however, it may not be a suitable marker as it only accounts for residual fecal butyrate following intestinal absorption. Therefore, we aimed to develop a novel quantitative system for evaluating intestinal butyrate production. We designed a primer set targeting the gene encoding butyryl-coenzyme A (CoA):acetate CoA-transferase (but), a key enzyme involved in butyrate synthesis. The new primer set specifically reacted with but-harboring bacteria, including Faecalibacterium, suggesting its suitability for the comprehensive and accurate detection of intestinal butyrate-producing bacteria. We established a sys

SUBMITTER: Hane M 

PROVIDER: S-EPMC12838319 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature