Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy.

ABSTRACT: Imaging living organisms with molecular selectivity typically requires the introduction of specific labels. Many applications in biology and medicine, however, would significantly benefit from a noninvasive imaging technique that circumvents such exogenous probes. In vivo microscopy based on vibrational spectroscopic contrast offers a unique approach for visualizing tissue architecture with molecular specificity. We have developed a sensitive technique for vibrational imaging of tissues by combining coherent anti-Stokes Raman scattering (CARS) with video-rate microscopy. Backscattering of the intense forward-propagating CARS radiation in tissue gives rise to a strong epi-CARS signal that makes in vivo imaging possible. This substantially large signal allows for real-time monitoring of dyna

SUBMITTER: Evans CL 

PROVIDER: S-EPMC1283840 | biostudies-literature | 2005 Nov

REPOSITORIES: biostudies-literature