Characterization of plasmalogen production in facultative anaerobic bacteria and aerobic synthesis in recombinant <i>Escherichia coli</i> expressing anaerobic bacterium-derived plasmalogen synthase genes.

ABSTRACT: Plasmalogens are glycerophospholipids with vital physiological functions, conferring antioxidant properties and contributing to membrane stabilization. While plasmalogen synthase genes plsA and plsR were identified in the obligate anaerobic bacterium Clostridium perfringens, plasmalogen production has not been reported in facultative anaerobes, in which a single gene commonly encodes plsA. To establish a cost-effective microbial plasmalogen production system, we screened 38 lactic acid bacterial strains and identified 11 plasmalogen producers, with Enterococcus faecalis K-4 exhibiting the highest productivity. Optimization of culture conditions, including the substitution of glucose with lactose and the addition of soy-derived peptides, increased plasmalo

SUBMITTER: Irimajiri R 

PROVIDER: S-EPMC12838417 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature