Dechlorination of mixed chlorinated organophosphate esters (V6 and TCEP) and associated reductive dehalogenase gene expression by <i>Dehalococcoides mccartyi</i>.

ABSTRACT: Dehalococcoides mccartyi (D. mccartyi) plays a critical role in the dechlorination of halogenated organic pollutants, yet its performance on mixed chlorinated organophosphate esters (Cl-OPEs) remains poorly understood. In this study, a mixed microbial culture containing D. mccartyi was developed to dechlorinate commercial V6, a mixture comprising 80.2% 2,2-bis(chloromethyl)trimethylene bis(bis(2-chloroethyl) phosphate) (V6), 9.4% tris(2-chloroethyl) phosphate (TCEP), and other Cl-OPE impurities. Within 10 days, 99.5% of TCEP and 95.4% of V6 were dechlorinated. The presence of TCEP slightly enhanced the dechlorination of V6 compared with V6 as a purified compound. Both compounds underwent cleavage of C-O and C-Cl bonds in their chloroethoxy groups (Cl-CH₂-CH<

SUBMITTER: Yang S 

PROVIDER: S-EPMC12838431 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature