Identification of Key Sequence Motifs Essential for the Recognition of m⁶A Modification in RNA.

ABSTRACT: N6-methyladenosine (m⁶A) constitutes the most prevalent nucleotide modification within eukaryotic messenger RNA (mRNA). Variations in m⁶A levels are associated with numerous human diseases and health conditions, including various forms of cancer, diabetes, neurological disorders, male infertility, and obesity. Nevertheless, the molecular mechanisms underpinning the recognition of m⁶A by different 'reader' proteins remain incompletely elucidated. In this study, we used phage display to identify key sequence features that methyl readers recognize in m⁶A. This study shows that m⁶A modifications affect the mRNA interactome. A peptide motif recognizing m⁶A in DRACH sequences suggests a common recognition mechanism, though protein

SUBMITTER: Mollah A 

PROVIDER: S-EPMC12838727 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature