Engineering a CRISPR-Mediated Dual Signal Amplification-Based Biosensor for miRNA Determination.

ABSTRACT: MicroRNAs, pivotal regulators of gene expression and physiology, serve as reliable biomarkers for early cancer diagnosis and therapy. As one of the earliest discovered miRNAs in the human genome, miRNA-21 provides critical information for early cancer diagnosis, drug therapy, and prognosis. In this work, we harness CRISPR as a bridge to integrate target-induced self-priming hairpin isothermal amplification (SIAM) with terminal transferase (TdT) polymerization labeling, constructing a facile, straightforward electrochemical biosensor for sensitive miRNA-21 detection. Unlike conventional single-strand template-based exponential amplification (EXPAR), the SIAM hairpin undergoes target triggered intramolecular conformational change, initiating extension and strand displacement reactions that s

SUBMITTER: Liang Z 

PROVIDER: S-EPMC12838993 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature