Microfluidic Chamber Design for Organ-on-a-Chip: A Computational Fluid Dynamics Study of Pillar Geometry and Pulsatile Perfusion.

ABSTRACT: Organ-on-a-Chip (OOC) platforms are microfluidic systems that recreate key features of human organ physiology in vitro via controlled perfusion. Fluid mechanical stimuli strongly influence cell morphology and function, making this important for cardiovascular OOC applications exposed to pulsatile blood flow. However, many existing OOC devices employ relatively simple chamber geometries and steady inflow assumptions, which may cause non-uniform shear exposure to cells, create stagnant regions with prolonged residence time, and overlook the specific effects of pulsatile perfusion. Here, we used computational fluid dynamics (CFD) to investigate how chamber geometry and inflow conditions shape the near-wall flow environment on a cell culture surface at a matched cycle-averaged volumetric flow

SUBMITTER: Liao A 

PROVIDER: S-EPMC12839026 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature