Highly Sensitive Biosensor for the Detection of Cardiac Troponin I in Serum via Surface Plasmon Resonance on Polymeric Optical Fiber Functionalized with Castor Oil-Derived Molecularly Imprinted Nanoparticles.

ABSTRACT: In this work, we report the development of a highly sensitive optical sensor for the detection of cardiac troponin I (cTnI), a key biomarker for early-stage myocardial infarction diagnosis. The sensor combines castor oil-derived biomimetic receptors, called GreenNanoMIPs and prepared via the molecular imprinting technology using as a template an epitope of cTnI (i.e., the NR10 peptide), with a portable multimode plastic optical fiber surface plasmon resonance (POF-SPR) transducer. For sensing, gold SPR chips were functionalized with GreenNanoMIPs as proven by refractive index changes and confirmed by means of XPS. Binding experiments demonstrated the cTnI_nanoMIP-SPR sensor's ability to detect both the NR10 peptide epitope and the full-length cTnI protein within minutes (t = 10 min), with

SUBMITTER: Marinangeli A 

PROVIDER: S-EPMC12839181 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature