Experimental Lung Ultrasound Scoring in a Murine Model of Aspiration Pneumonia: Challenges and Diagnostic Perspectives.

ABSTRACT: Background: Aspiration pneumonia (AP) remains a major cause of morbidity and mortality, yet non-invasive tools for monitoring lung injury in preclinical models are limited. Lung ultrasound (LUS) is widely used clinically, but existing murine scoring systems lack anatomical resolution and have not been validated for aspiration-related injury. Methods: We developed the Modified Lung Edema Ultrasound Score (MLEUS), a region-structured adaptation of the Mouse Lung Ultrasound Score (MoLUS), designed to accommodate the heterogeneous and gravity-dependent injury patterns characteristic of murine AP. Male C57BL/6 mice were assigned to sham, 6 h, 24 h, or 48 h groups. Regional LUS findings were compared with histological injury scores and wet-to-dry (W/D) ratios. Inter-rater reliabili

SUBMITTER: Chuang CW 

PROVIDER: S-EPMC12839569 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature