Transcriptomic Profiling of Cutibacterium acnes IA₁-Infected Keratinocytes Reveal Hub Genes and CLR Pathway in Acne Pathogenesis.

ABSTRACT: Acne vulgaris is a prevalent chronic inflammatory skin disorder affecting over 85% of adolescents. Emerging evidence indicates that Cutibacterium acnes phylotype IA₁ contributes to acne initiation and progression, yet its precise mechanisms in epidermal keratinocytes remain unclear. This study investigated C. acnes IA1's effects on keratinocyte behavior using an in vitro HaCaT cell model. Cells were co-cultured with live C. acnes IA₁ (CICC 10864) for 24 h. Transcriptomic profiling identified 769 differentially expressed genes (DEGs; adjusted p < 0.05, |log2FC| > 1), including 392 upregulated and 377 downregulated. The protein-protein interaction network analysis via Cytoscape revealed key hub genes (HNRNPA2B1, HNRNPM, RBM39). Enrichment ana

SUBMITTER: Li J 

PROVIDER: S-EPMC12839951 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature