Influence of Extraction Method on the Bioactivity of Pistacia vera L. Extracts: Metabolic and Gene Expression Changes in Colorectal Cancer Cells.

ABSTRACT: Sustainable extraction methods represent a key strategy in green chemistry and nutraceutical development, aiming to replace conventional solvent-based techniques while maintaining extract quality and safety. This study compared pistachio (Pistacia vera L.) extracts obtained by Ultrasound-Assisted Extraction (UAE) and a classical solvent-based protocol, focusing on compositional features and biological effects. Extracts were characterized for their chemical profiles, and their impact on HCT-116 colon-derived cells was evaluated through viability assays and gene expression analysis. The UAE-derived extract, richer in carbohydrates, promoted higher cell proliferation after 72 h, whereas the classical extract upregulated HMOX-1, suggesting activation of antioxidant defense pathwa

SUBMITTER: Zerbo G 

PROVIDER: S-EPMC12840026 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature