Methodological and Short-Term Diurnal Variation in Surface and Cargo Proteins in Plasma Extracellular Vesicles.

ABSTRACT: Extracellular vesicles (EVs) are known as potential biomarkers for several diseases; nevertheless, the degree of technical and biological variability is not yet adequately characterized. Because pre-analytical factors such as blood collection time and EV subpopulation could confound biomarker studies, we performed a pilot study systematically quantifying methodological and biological variability including EV-Array (surface proteins), and proteome characterization of cargo. Plasma samples from six healthy adults were collected at two time points (morning and afternoon) and plasma was analyzed with EV-Array, and isolated EVs were analyzed using nanoparticle tracking analysis (NTA), and label-free mass spectrometry (LC-MS/MS). Methodological repeatability was high for NTA particle size (3.3%

SUBMITTER: Krzyslak H 

PROVIDER: S-EPMC12840092 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature