Nucleolar Cdc14 Splitting Reflects Recombination Context and Meiotic Chromosome Dynamics.

ABSTRACT: Chromosome dynamics, recombination, and nucleolar organization intersect during meiotic prophase I, yet how the recombination context influences nucleolar architecture remains unclear. We analyzed the nucleolar pool of Cdc14 in Saccharomyces cerevisiae under matched prophase I gating and a uniform, frame-based operational definition of transient two-focus episodes. In a prophase-arrest reference, Cdc14-mCherry formed a predominant single nucleolar focus with occasional, reversible two-focus episodes that Nop56-GFP placed within the nucleolar compartment (nucleolar splitting). Splitting rose sharply when interhomolog recombination was compromised and remained elevated when Spo11 catalytic activity was abolished, indicating that increased DSB formation is not required and pointing ins

SUBMITTER: Rodriguez-Jimenez P 

PROVIDER: S-EPMC12841278 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature