Immortalization and Targeted Enrichment of HIV-Infected CD4⁺ T-Cells from Patients Under Antiretroviral Therapy.

ABSTRACT: Defective HIV-1 proviruses harboring mutations and/or large internal deletions represent the majority of HIV-1 sequences found in circulating peripheral blood mononuclear cells of people living with HIV with viremia suppressed by combination antiretroviral therapy; indirect evidence suggests that such sequences are transcriptionally active and may contribute to immune activation. In this study, we present a new approach allowing for high-efficiency screening, immortalization, and targeted enrichment of HIV-positive CD4⁺ T-cells isolated from people living with HIV. Using this method, we were able to isolate and expand patient-derived cells, identify mutations and deletions via sequencing, and confirm that those proviruses were transcriptionally and translationally active in vitr

SUBMITTER: Bruchey WE 

PROVIDER: S-EPMC12841691 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature