Molecular Identification and Pathogenicity of a Chilli Veinal Mottle Virus Isolate from Sichuan, China.

ABSTRACT: Chilli veinal mottle virus (ChiVMV) is an important potyvirus that poses a serious threat to crop production. In this study, small RNA sequencing and molecular cloning were used to obtain the complete genome sequence of a ChiVMV isolate identified in pepper plants in Sichuan (SC1 isolate). Molecular evolutionary and phylogenetic analysis of SC1 and 35 ChiVMV isolates revealed four clades of ChiVMV isolates. Recombination analysis found 23 recombinant events and 28 recombinants, with the SC1 isolate arising from the recombination of the PK isolate from Pakistan and the YNpe isolate from Yunnan, China. A full-length infectious cDNA clone of ChiVMV was constructed and demonstrated to be infectious in both Nicotiana benthamiana and pepper plants. Moreover, a Myc-tag was inserted after N

SUBMITTER: Chen C 

PROVIDER: S-EPMC12841742 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature