Functional Analysis of the Scarlet Gene in the Cricket Gryllus bimaculatus.

ABSTRACT: The scarlet gene encodes an ATP-binding cassette transporter involved in eye pigmentation across various insect species. In this study, we functionally characterized the scarlet homolog (Gbst) in the cricket Gryllus bimaculatus, a hemimetabolous model organism. Clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9-mediated knockout of Gbst generated a stable yellow-eyed mutant line (Gbst^-/-) with changed pigmentation evident from embryogenesis through adulthood. Quantitative real-time PCR analysis showed that scarlet expression was extremely low in Gbst^-/-, and the transcript levels of white and brown were also reduced. Histological sections of the compound eyes show

SUBMITTER: Zeng LF 

PROVIDER: S-EPMC12842043 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature