Molecular Evaluation of Different Enrichment Methods for Extracellular Vesicles from Healthy Subjects' Biobanked Serum.

ABSTRACT: Extracellular vesicles (EVs) from human body fluids are valuable tools for biomarker discovery and for exploring the mechanisms underlying various pathologies, including infectious diseases. The translation of EV research into clinical practice is however hindered by the variability in EV pre-clinical investigations. Therefore, standardisation of analytical procedures and reporting policies is essential. Human serum is a key biological matrix for biomarker discovery and is commonly stored within biobanks. Here, we investigated different strategies for EV enrichment from small volumes of biobanked serum and evaluated their impact on EVs' downstream analyses. EVs were obtained from 250 μL of biobanked serum using ultracentrifugation (UC), size-exclusion chromatography-based methods (ExoSpin-

SUBMITTER: Deiana M 

PROVIDER: S-EPMC12842214 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature