Proteomic profiling of porcine seminal extracellular vesicles reveals potential in vivo fertility biomarkers.

ABSTRACT:

Background

Predicting male fertility in farm animals remains a challenge. Seminal plasma (SP) contains a high amount of heterogeneous seminal extracellular vesicles (sEVs), believed involved in reproductive processes and maybe key to understanding male fertility.

Aims

To identify the sEV proteins that are differentially expressed between more and less fertile boars and that could be candidates for fertility biomarkers in boars used in artificial insemination (AI) programs.

Materials and methods

Small (S) and large (L) sEV subsets from SP samples of AI boars with differences in fertility: high (H) or low (L) farrowing rate (FR) and large (L) or small (S) litter size (LS). The S- and L-sEV subsets were isolated by size exclusion chromatography and characterized accordin

SUBMITTER: Barranco I 

PROVIDER: S-EPMC12842868 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature