Distinction Between Aspergillus oryzae and Aflatoxigenic Aspergillus flavus by Rapid PCR Method Based on the Comparative Sequence Analysis of the Aflatoxin Biosynthesis Gene Cluster.

ABSTRACT: Aspergillus oryzae and Aspergillus flavus are closely related species within the Aspergillus section Flavi, sharing approximately 99.5% genomic similarity. Despite this similarity, they differ markedly in their ability to produce aflatoxin, a carcinogenic mycotoxin synthesized by the aflatoxin biosynthesis gene cluster (ABGC). Species and strains included within section Flavi display diverse deletion patterns in the ABGC at the sequence level. In this study, we performed an in-depth comparative analysis of the ABGC of 30 strains belonging to section Flavi, including isolates obtained from nuruk. The analysis revealed that A. oryzae exhibits distinct large-scale or locus-specific deletions in the ABGC compared to other related species.

SUBMITTER: Jeong E 

PROVIDER: S-EPMC12842993 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature