Multi-Omics Analyses Reveal the Antifungal Mechanism of Phenazine-1-Carboxylic Acid Against Pseudogymnoascus destructans.

ABSTRACT: White-nose syndrome (WNS) is an infectious disease of bats caused by the psychrophilic fungus Pseudogymnoascus destructans. Phenazine-1-carboxylic acid (PCA) is a microbial secondary metabolite with broad-spectrum antifungal activity. Previous studies show that PCA suppresses the growth of P. destructans at low concentrations, yet its mechanism remains unclear. Here, we evaluated the in vitro antifungal activity of PCA. We then investigated its potential mechanism using physiological and biochemical assays, as well as integrated transcriptomic and metabolomic analyses. PCA showed effective antifungal activity against P. destructans (EC₅₀ = 32.9 μg/mL). Physiological and biochemical assays indicated that PCA perturbed cell wall organization and increased memb

SUBMITTER: Huang Z 

PROVIDER: S-EPMC12843306 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature