Zinc-Finger 5 Is an Activation Domain in the Saccharomyces cerevisiae Stress-Responsive Transcription Factor Fzf1.

ABSTRACT: Fzf1 is a Saccharomyces cerevisiae transcription factor that contains five zinc finger domains (ZF1-5) and induces the expression of at least five genes in response to various chemical stresses by recognizing the shared promoter consensus sequence CS2. The N-terminal ZF1-3 are required and sufficient for binding to CS2, while ZF4 negatively regulates the activity of Fzf1. However, the effect of ZF5 on the activity of Fzf1 is not well defined. In this study, substitutions of the two zinc-coordinating Cys residues (C248S and C253S) of ZF5, or deletion of the whole ZF5 domain, compromised the chemical stress-induced activation of Fzf1. Since the elevated Fzf1-regulated gene expression caused by fzf1-ZF4 could also be reversed by additional deletion of ZF5 or C248S/C253S substitu

SUBMITTER: Du Y 

PROVIDER: S-EPMC12843318 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature